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Supplementation of these factors in nonhepatic human cell lines enabled HCV replication and particle production.
Vero cells established from monkey kidney are commonly used for the production of vaccines against a variety of viruses.
In this study, we aimed to establish a novel Vero cell line to reconstruct the HCV life cycle.
Unmodified Vero cells did not allow HCV infection or replication.
For example, HEK293T cells are human kidney-derived cells, and the ectopic expression of micro RNA 122 (mi R-122) and Claudin-1 (CLDN1) enabled HCV replication after HCV infection of HEK293T cells (16).